Comprehensive Guide to Fluorescence Analyzer: Operating Procedures, Faults, and Repair Methods

I. Overview and Basic Principles of Fluorescence Analyzer

The fluorescence analyzer is a precise instrument that utilizes the fluorescence phenomenon of substances under specific excitation conditions to analyze sample composition and properties. When chemicals in a sample are irradiated by light of a specific wavelength, they absorb energy, transition to a higher energy state, and subsequently return to the ground state while releasing photons, producing fluorescence. By measuring the intensity and characteristic spectrum of fluorescence, the fluorescence analyzer can accurately analyze the composition and structure of the sample.

II. Operating Procedures for Fluorescence Analyzer

Startup Preparation:

• Ensure the normal operation of external equipment such as air compressor, water cooler, and gas supply system.
• Turn on the fluorescence analyzer host and computer, and run the analysis software.

Sample Preparation:

• Prepare samples according to experimental requirements, ensuring sample purity and compatibility with reagents.
• Place the sample in an appropriate container for testing.

Instrument Calibration:

• Perform zero-point calibration to ensure accurate measurements.
• Adjust instrument parameters as needed, such as excitation wavelength and emission wavelength.

Sample Testing:

• Place the sample in the sample chamber of the fluorescence analyzer.
• Initiate the testing program and wait for the instrument to complete the measurement of the fluorescence spectrum.
• Record and analyze the fluorescence spectrum data to draw conclusions about the sample’s composition and properties.

Shutdown and Cleaning:

• After testing, turn off the fluorescence analyzer host and computer.
• Clean the sample chamber and the exterior of the instrument to maintain its good condition.

III. Types of Faults and Repair Methods for Fluorescence Analyzer

Unstable Fluorescence Intensity:

• Causes: Aging of lamp filament, dirty electrodes, fluctuations in ambient temperature.
• Repair Methods: Replace the lamp filament, clean the electrodes, stabilize the ambient temperature.

Decreased Sensitivity:

• Causes: Expired reagents, incompatibility between sample and reagent, contamination of the optical path system.
• Repair Methods: Replace with fresh reagents, test using the same type of sample, clean the optical path system.

Instrument Failure to Turn On:

• Causes: Power supply failure, connection issues between computer and host, software system errors.
• Repair Methods: Check the power supply, reconnect the computer and host, restart the software system or contact the manufacturer for repair.

Low Water Flow or Water Circuit Blockage:

• Causes: Blocked water filter or impurities in the water circuit system.
• Repair Methods: Clean or replace the water filter, inspect and clear impurities in the water circuit system.

IV. Repair Precautions

• Before performing any repair operations, be sure to turn off the instrument’s power supply to ensure safety.
• Use professional tools for disassembly and installation to avoid damaging instrument components.
• Follow the repair manuals and guides provided by the manufacturer to ensure correct repair procedures.
• For complex faults or issues that cannot be resolved independently, promptly contact professional repair personnel from Rongji Electromechanical Company.

V. Conclusion

As a high-precision analytical instrument, the fluorescence analyzer plays a crucial role in scientific research and production fields. Through this introduction, we have gained an understanding of the basic principles, usage methods, common fault types, and repair methods of the fluorescence analyzer. We hope that this information will help you better use and maintain the fluorescence analyzer, thereby improving research and production efficiency.

VI. Brands and Models of Fluorescence Analyzers Repaired by Rongji Electromechanical Company

1. Thermo Fisher Scientific
   • Fluoroskan FL: Microplate Fluorometer
   • NanoDrop 3300: Micro-Volume Fluorometer
   • Lumina Fluorescence Spectrometer: General Fluorescence Spectrometer
2. Agilent Technologies
   • Cary Eclipse: Fluorescence Spectrometer
   • Agilent 4300 Handheld FTIR: Handheld Fluorescence Analyzer
3. PerkinElmer
   • LS 55: Fluorescence Spectrometer
   • EnVision: Multimode Microplate Reader
   • Victor Nivo: Multimode Microplate Reader
4. Horiba Scientific
   • FluoroMax-4: Fluorescence Spectrometer
   • FluoroMax Plus: High-Performance Fluorescence Spectrometer
   • Aqualog: Water Quality Analysis Fluorescence Spectrometer
5. Shimadzu
   • RF-6000: High-Sensitivity Fluorescence Spectrometer
   • RF-5301PC: Fluorescence Spectrometer
6. Edinburgh Instruments
   • FLS1000: Fluorescence Spectrometer
   • FS5: Fluorescence Spectrometer
   • Mini-tau: Fluorescence Lifetime System
7. Hitachi
   • F-7000: Fluorescence Spectrometer
   • F-7100: High-Performance Fluorescence Spectrometer
   • F-2700: Compact Fluorescence Spectrometer
8. Zeiss
   • LSM 880: Laser Scanning Confocal Microscope with Fluorescence Analysis Function
   • Axio Imager: Fluorescence Microscope
9. Bruker
   • FOCUS-G: Portable Fluorescence Spectrometer
   • CRYO-G: Fluorescence Lifetime Microscope
10. Tecan
    • Spark: Multimode Microplate Reader with Fluorescence Analysis Function
    • Infinite 200 PRO: Multimode Microplate Reader
11. BioTek (Agilent)
    • Cytation 5: Multimode Microplate Reader and Imaging System
    • Synergy H1: Multimode Microplate Reader
12. Molecular Devices
    • SpectraMax i3x: Multimode Microplate Reader
    • FlexStation 3: Fluorescence Analysis System
13. Photon Technology International (PTI)
    • QuantaMaster 8000: Fluorescence Spectrometer
    • TimeMaster: Fluorescence Lifetime System
14. Jobin Yvon (Horiba)
    • FluoroLog: Fluorescence Spectrometer
    • FluoroCube: Fluorescence Lifetime System

Rongji Electromechanical Company has nearly 30 years of experience in repairing fluorescence analyzers (fluorescence spectrometers, atomic fluorescence spectrometers) and can quickly repair various types of instruments. Additionally, we recycle and sell various used fluorescence analyzers. Welcome to consult.